TY - JOUR
T1 - Three dimensional principal component analysis used for the study of enzyme kinetics
T2 - An empirical approximation for the determination of the dimensions of component matrices
AU - Morais, Helena
AU - Ramos, Cristina
AU - Forgács, Esther
AU - Jakab, Annamaria
AU - Cserháti, Tibor
AU - Oliviera, José
AU - Illés, Tibor
AU - Illés, Zoltán
PY - 2001
Y1 - 2001
N2 - The effect of cation type and concentration and fermentation time on the xylanase production of four strains of Lentinus edodes was determined. Three dimensional principal component analysis (3D-PCA) followed by non-linear mapping technique (NLMAP) was employed for the assessment of similarities and dissimilarities between the enzymatic activities. An empirical method was developed for the reduction of the dimensionality of component matrices. No linear relationship was found between the effect of cations on the enzyme production and their ion radii, concentration and charge. Enzyme production was similar up till 20 days of fermentation then changed considerably. The enzyme activities of strains also showed marked differences. The results proved that the reduction of the dimensionality of component matrices exerts a negligible effect on the variance explained but it modifies the similarities and dissimilarities among the elements of the original matrix. It was established that 3D-PCA followed by NLMAP is a valuable tool for the evaluation of three dimensional data matrices in enzyme kinetic studies.
AB - The effect of cation type and concentration and fermentation time on the xylanase production of four strains of Lentinus edodes was determined. Three dimensional principal component analysis (3D-PCA) followed by non-linear mapping technique (NLMAP) was employed for the assessment of similarities and dissimilarities between the enzymatic activities. An empirical method was developed for the reduction of the dimensionality of component matrices. No linear relationship was found between the effect of cations on the enzyme production and their ion radii, concentration and charge. Enzyme production was similar up till 20 days of fermentation then changed considerably. The enzyme activities of strains also showed marked differences. The results proved that the reduction of the dimensionality of component matrices exerts a negligible effect on the variance explained but it modifies the similarities and dissimilarities among the elements of the original matrix. It was established that 3D-PCA followed by NLMAP is a valuable tool for the evaluation of three dimensional data matrices in enzyme kinetic studies.
KW - Lentinus edode strains
KW - Three dimensional principal component analysis
KW - Xylanase production
UR - http://www.scopus.com/inward/record.url?scp=0035155653&partnerID=8YFLogxK
U2 - 10.1002/1521-3838(200110)20:3<241::AID-QSAR241>3.0.CO;2-D
DO - 10.1002/1521-3838(200110)20:3<241::AID-QSAR241>3.0.CO;2-D
M3 - Article
AN - SCOPUS:0035155653
SN - 0931-8771
VL - 20
SP - 241
EP - 247
JO - Quantitative Structure-Activity Relationships
JF - Quantitative Structure-Activity Relationships
IS - 3
ER -