TY - JOUR
T1 - Interaction of hydroxypropyl-β-cyclodextrin with peptides, studied by reversed-phase thin-layer chromatography
AU - Gere-Pászti, E.
AU - Cserháti, T.
AU - Forgács, E.
AU - Deyl, Z.
AU - Miksik, I.
AU - Eckhardt, A.
AU - Illés, Z.
PY - 2005
Y1 - 2005
N2 - The effect of various concentrations of hydroxypropyl-β-cyclodextrin (HP-β-CD) on the reversed-phase thin-layer chromatographic mobility of some low molecular mass peptides was measured in distilled water and in 0.05 M of LiCl, NaCl, KCl, RbCl, and CsCl solutions, and the relative strength of HP-β-CD-peptide interaction has been calculated from the dependence of retention on the concentration of HP-β-CD in the mobile phase. Lipophilicity of the peptides decreased regularly with increasing concentration of HP-β-CD in the mobile phase, proving the interaction (probably the formation of inclusion complexes) between peptides and HP-β-CD. Principal component analysis indicated that each salt influenced the interaction differently. Peptides formed clusters on the two-dimensional nonlinear map according to the character of the amino acid monomer and not according to the number of amino acid units, suggesting that only one terminal amino acid is included in the cavity of HP-β-CD.
AB - The effect of various concentrations of hydroxypropyl-β-cyclodextrin (HP-β-CD) on the reversed-phase thin-layer chromatographic mobility of some low molecular mass peptides was measured in distilled water and in 0.05 M of LiCl, NaCl, KCl, RbCl, and CsCl solutions, and the relative strength of HP-β-CD-peptide interaction has been calculated from the dependence of retention on the concentration of HP-β-CD in the mobile phase. Lipophilicity of the peptides decreased regularly with increasing concentration of HP-β-CD in the mobile phase, proving the interaction (probably the formation of inclusion complexes) between peptides and HP-β-CD. Principal component analysis indicated that each salt influenced the interaction differently. Peptides formed clusters on the two-dimensional nonlinear map according to the character of the amino acid monomer and not according to the number of amino acid units, suggesting that only one terminal amino acid is included in the cavity of HP-β-CD.
KW - Cyclodextrin
KW - Nonlinear map
KW - Peptides
KW - TLC
UR - http://www.scopus.com/inward/record.url?scp=26444482368&partnerID=8YFLogxK
U2 - 10.1080/10826070500189877
DO - 10.1080/10826070500189877
M3 - Article
AN - SCOPUS:26444482368
SN - 1082-6076
VL - 28
SP - 2619
EP - 2632
JO - Journal of Liquid Chromatography and Related Technologies
JF - Journal of Liquid Chromatography and Related Technologies
IS - 16
ER -